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Endogenous flavin content in spheroplasts and mitochondria.

Journal: BioMed Research International

Article Title: Alteration of ROS Homeostasis and Decreased Lifespan in S. cerevisiae Elicited by Deletion of the Mitochondrial Translocator FLX1

doi: 10.1155/2014/101286

Figure Lengend Snippet: Endogenous flavin content in spheroplasts and mitochondria.

Article Snippet: Rf, FMN, and FAD content in spheroplasts and SCM was measured in aliquots (5–80 μ L) of neutralized perchloric extracts by means of HPLC (Gilson HPLC system including a model 306 pump and a model 307 pump equipped with a Kontron Instruments SFM 25 fluorometer and Unipoint system software), essentially as previously described [ ].

Techniques: